Anti-CD39 (Apyrase, ATPDase, EC3.6.1.5, Ecto-Apyrase,Ecto-ATPase, ENTPD1, Ectonucleoside triphosphat

Ectonucleoside triphosphate diphosphohydrolase-1 also known as CD39 or NTPDase-1, ENTPD1 was originally described as a B lymphocyte cell surface marker, but it is also present on the surface of natural killer cells, T cells, and some endothelial cells. ENTPD1 hydrolyzes the beta- and gamma phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, ENTPD1 plays a role in the regulation of purinergic signaling. ENTPD1 is also involved in the processes of thromboregulation and vascular inflammation. The amino acid sequence of mouse ENTPD1 is 77% and 72% identical to that of human and canine. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells using mouse splenocytes, Western Blot: 1ug/ml, Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant mouse CD39, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.