Anti-CD3, epsilon (Early T Cell Marker), Phycoerythrin/Cy5 conjugated, clone 5K2

CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. In the presence of Fc receptor-bearing accessory cells, soluble antibody can activate primed and naïve T cell in vitro. It can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL's. Immobilized antibody can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by this antibody may result in apoptotic cell death (1-6). Applications:  Identification and enumeration of CD3+ cells by flow cytometry 1, 2,  Immunoprecipitation 1,  Western blotting 7,  In vitro depletion of CD3+ cells 2,  In vitro activation of T cells 2-4,  Immunohistochemistry 8, Recommended Dilution: Flow Cytometry: < 1ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer., , PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.