Anti-CD244 [2B4] (human), mAb (ANC244.8/8F7) (preservative free)

Isotype: Mouse IgG1kappa Source/Host: Purified from concentrated hybridoma tissue culture supernatant. Immunogen/Antigen: Recombinant soluble human CD244. Label/Conjugates: Preservative Free Application: ELISA Flow Cytometry Crossreactivity: Human Specificity: Antibody ANC244.8 binds to recombinant CD244-muIg (#ANC-544-020) in EIA, and to cell surface CD244 on human PBL in FACS. ANC244.8 binds to a CD244 epitope that is distinct from the binding sites of clone ANC2B4/3B12 (#ANC-259-020). It blocks binding of clone C1.7. Purity: >95% (SDS-PAGE) Purity Detail: Protein A purified. Concentration: 1.0mg/ml Formulation: 50mM Sodium Phosphate pH 7.5, 100mM KCl, 150mM NaCl. Isotype Negative Control: Mouse IgG1 Isotype Control CD244 (2B4, SLAMF4) is a 66kDa member of the CD2/SLAM related receptor family (SRR). It is expressed on NK, T cell subsets, monocytes and eosinophils. It is expressed at high levels on leuikemia initiating cells, and plays a role in their proliferative potential. In mice there are two transcription variants, a long form and a short form of CD244 each of which have distinct signaling properties. In humans, only the long form is expressed. Decreased CD244 expression on monocytes of SLE patients correlated (negatively) with autoantibody titer. CD244 mediated function in immune response can vary greatly dependant on the context of its ligation.