Anti-CD23 (IgE Receptor gp45), FITC conjugated, clone 2Q1609

The CD23 antigen is the low affinity IgE Fc receptor, which is a 49kD protein with 38 and 28kD fragments (1). It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages and platelets. It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation. The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors. CD23 has been described as a membrane bound cytokine,"" in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and , Differentiation of B cell subpopulations by cell sorting. Other applications not tested. Recommended Dilution: Flow Cytometry: < 1ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. , "