Anti-CD178 (Apoptosis Antigen Ligand, APT1LG1, APTL, CD95 Ligand, CD95L, CD95-L, FAS Antigen Ligand,

The FAS ligand (Fas-L) belongs to the tumor necrosis factor family. The full length Fas-L protein has a cytoplasmic domain, transmembrane (Type II) domain and extracellular domain. The membrane-bound form of Fas-L is proteolytically cleaved to produce soluble Fas-L. There are at least 2 known isoforms of Fas-L produced by alternate splicing. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. , Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry (paraffin and formalin): 1-2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.