Anti-CD119 (CD119 Antigen, CDw119, FLJ45734, Immune Interferon Receptor 1, Interferon gamma Receptor

The high-affinity IFN-G receptor complex is made up of two type I membrane proteins, IFN-G R1 (IFN-G Ra) and IFN-G R2 (IFN-G Rb). Both proteins are members of the type II cytokine receptor family and share ~52% overall sequence identity. IFN-G R1 is the ligand-binding subunit that is necessary and sufficient for IFN-G binding and receptor internalization. IFN-G R2 is required for IFN-G signaling but does not bind IFN-G by itself. Human IFN-G R1 cDNA encodes a 499aa residue protein with a 17aa signal peptide, a 228aa extracellular domain, a 23aa transmembrane domain, and a 221aa intracellular domain. Human and mouse IFN-G R1 share 52aa sequence similarity and bind IFN-G in a species-specific manner. IFN-G R1 is constitutively expressed in most cell types. Soluble IFN-G R1 that binds IFN-G has been detected in biological fluids. The recombinant soluble IFN-G R1 has been shown to bind IFN-G with high affinity and is a potent IFN-G antagonist. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 0.1-0.4ug/ml, ELISA (capture): 2-8ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.