Anti-CD104 (Integrin beta4)

Anti-CD104 (Integrin beta4)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
C2446-01H.100 100 µg - -

3 - 19 business days*

916.00€
 
CD104 (also GP150 and Integrin beta 4) is a 150-200kD member of the integrin beta family. It... more
Product information "Anti-CD104 (Integrin beta4)"
CD104 (also GP150 and Integrin beta 4) is a 150-200kD member of the integrin beta family. It forms noncovalent heterodimers with integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human integrin beta 4 is a type I transmembrane glycoptotein that is 1795 amino acids (aa) in length. It contains a 683aa extracellular domain (ECD) (aa28-710) and a large 1089aa cytoplasmic region. Two ECD alternate splice forms may exist. One shows an 84aa substitution for the 84aa between aa21-704. A second shows a 144aa substitution for the first 704aa. Human beta 4 ECD shares 88% aa sequence identity with mouse beta4 ECD. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml, Using a colorimetric detection system, the detection limit for recombinant human Integrin 4 is ~25ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold., Flow Cytometry: 0.2mg/ml, 10ul labels 1-2.5x10e5 human A431 cells in a total reaction volume <200ul. , Immunocytochemistry: 10ug/ml detects CD104 in A431 human epidermoid carcinoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted C2446-01H overnight at 4ºC followed by anti-mouse IgG (FITC) at RT in the dark for 1 hour. Cells were washed with PBS, 0.1% BSA between each step., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: Anti-GP150, Anti-ITGB4, Anti-CD104, Anti-Integrin beta-4
Supplier: United States Biological
Supplier-Nr: C2446-01H

Properties

Application: ELISA, FC, ICC, WB
Antibody Type: Monoclonal
Clone: 7H253
Conjugate: No
Host: Mouse
Species reactivity: human
Immunogen: CHO-derived recombinant human Integrin Beta 4 (aa28-710, Accession # P16144)
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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