Anti-CCDC88A (Girdin, Akt Phosphorylation Enhancer, APE, Coiled-coil Domain-containing Protein 88A,

Girdin is a Galpha-interacting protein that can enhance the activation of the protein kinase Akt, remodel the actin cytoskeleton, and is thought to be involved in the regulation of cell migration and cancer metastasis. It has recently been shown that Girdin interacts with Disrupted-in-Schizophrenia 1 (DISC1), a susceptibility gene for major psychiatric disorders. DISC1 is thought to be involved in the migration, positioning and differentiation of dentate granule cells (DGCs) during development, depletion of Girdin or blocking the Girdin-DISC1 interaction results in defects in axonal sprouting in the CA3 region of the hippocampus and overextended migration of mispositioning of DGCs, suggesting the Girdin plays a role in postnatal neurogenesis in the dentate gyrus. Applications: Suitable for use in Immunohistochemistry/Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry/Immunofluorescence: 20ug/ml, Western Blot: 1-2ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Rat Brain Tissue Lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.