Anti-CCBL1 (Glutamine Transaminase K, KAT1, KATI, KAT-I, Kyneurenine Aminotransferase I, Cysteine Co

CCBL1 (Cysteine Conjugate beta­Lyase 1, also GTK and KAT­I) is a 90­120kD homodimeric member of the Class I Pyridoxyl­phosphate­dependent aminotransferase family of enzymes. It is widely expressed, being found in fibroblasts, renal epithelium, hepatocytes, neurons and astrocytes. CCBL1 has multiple activities, including converting prodrugs into active formulations, generating kynurenic acid from kynurenine, thereby antagonizing NMDA receptors (but not likely presynaptic alpha 7 AChRs), and blocking the release of proinflammatory cytokines by microglia via GPCR­35 antagonism. Human CCBL1 is 422 amino acids (aa) in length, and contains one aspartate aminotransferase­like region (aa31­417). There are three potential splice variants, one that shows a deletion of aa68­117, a second that shows a deletion of aa251­422, and a third that contains a 21aa substitution for aa230­250. While the 422aa isoform is considered cytosolic, there is the potential for a 34aa N­terminal extension that would act as a mitochondrial targeting sequence. Such a sequence is reported for rat and nonhuman primate. Over aa182­422, human CCBL1 shares 82% aa sequence identity with mouse CCBL1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.