Anti-CBX6 / Chromobox protein homolog 6

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. CBX6 antibody detects Chromobox protein homolog 6, a member of the chromobox family within the Polycomb group (PcG) proteins, which play a critical role in transcriptional repression and chromatin remodeling. The full UniProt recommended name is Chromobox protein homolog 6 (CBX6), with older or alternate designations including chromobox homolog 6, Polycomb group protein chromobox 6, and human homolog of Drosophila Polycomb protein. CBX6 is a nuclear protein involved in maintaining the repressed state of genes through the formation of polycomb repressive complexes (PRCs), particularly PRC1, which works in conjunction with PRC2 to regulate histone methylation states and gene silencing across development and cell differentiation.CBX6 binds to trimethylated histone H3 at lysine 27 (H3K27me3), a histone modification mark associated with gene repression, serving as a reader of histone methylation marks and recruiting additional factors to maintain chromatin compaction. It contributes to establishing heritable transcriptional repression, ensuring stable lineage-specific expression patterns in embryonic stem cells and adult tissues. Dysregulation of CBX6 antibody targets, particularly in the Polycomb family, has been associated with aberrant cell proliferation and oncogenic transformation. Overexpression or misexpression of CBX6 has been observed in several cancer types, including hepatocellular carcinoma, prostate cancer, and gliomas, where it may influence epithelial-mesenchymal transition (EMT) and cancer stemness features.In molecular biology and epigenetic research, CBX6 antibody is widely used for immunofluorescence, chromatin immunoprecipitation (ChIP), and western blot assays to examine Polycomb protein localization and function. It helps distinguish CBX6 from other chromobox homologs (CBX2, CBX4, CBX7, CBX8), each of which has distinct but sometimes overlapping genomic binding profiles and developmental roles. CBX6 has been reported to modulate PRC1 recruitment in concert with histone ubiquitination factors such as RNF2 and BMI1, and to act as a chromatin structural modulator linking histone marks to gene repression. The protein is essential in maintaining pluripotency, regulating HOX gene clusters, and ensuring proper cell fate decisions.CBX6's gene, CBX6, is located on chromosome 22q13.1. It encodes a protein of approximately 412 amino acids, containing a conserved chromodomain responsible for histone binding and a C-terminal region that mediates protein-protein interactions within the PRC complex. Expression is ubiquitous but higher in testis, placenta, and certain cancerous tissues. Loss-of-function mutations or altered methylation of the CBX6 promoter region can disrupt PRC targeting, influencing gene activation programs and tumor suppressor networks. Studies also show CBX6 modulates long noncoding RNA (lncRNA)-mediated gene silencing, including interactions with lncRNAs such as HOTAIR and MALAT1, further linking CBX6 to chromatin topology and transcriptional memory.Because of its functional importance, CBX6 serves as a key biomarker in epigenetic and oncogenic signaling studies. NSJ Bioreagents provides validated reagents for detecting CBX6 in tissues across multiple applications including immunohistochemistry, immunoprecipitation, and ChIP-seq.