Anti-Cartilage Intermediate-Layer Protein 1 (CILP-1)

The CILP-1 (cartilage intermediate-layer protein 1) gene product is a monomeric glycoprotein precursor of two secreted, proteolytically generated products, a 90kD N-terminal CILP-1, and a 62kD C-terminal NTPPHase-homolog. It is found in both hyaline and fibrocartilage. Although the C-terminal NTPPHase-homolog (aa 725-1184) does not show enzyme activity, it may participate in matrix structural integrity. Human CILP-1 C-terminus shares 93% and 94% aa identity with porcine and mouse CILP-1 C-terminus, respectively. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhCILP-1 is ~ 1.3ng/well., Western Blot: 0.1-0.2ug/ml detection limit for rhCILP-1 is ~ 20ng/lane under non-reducing and reducing conditions., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.