Anti-CARD10 / Caspase recruitment domain-containing protein 10

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. CARD10 antibody detects Caspase recruitment domain-containing protein 10, a cytoplasmic scaffold protein encoded by the CARD10 gene on chromosome 22q13.1. CARD10 is part of the membrane-associated guanylate kinase (MAGUK) family and functions as an adaptor linking G protein-coupled receptor (GPCR) and receptor tyrosine kinase signaling to NF-kappaB activation. It is expressed in epithelial cells, endothelial cells, and some immune tissues, where it mediates signal transduction leading to cell survival, cytokine production, and inflammation.CARD10 operates as a component of the CARD10-BCL10-MALT1 (CBM) signaling complex. Upon activation of upstream receptors such as GPCRs or EGFR, CARD10 recruits BCL10 and MALT1, initiating downstream IKK complex activation and NF-kappaB signaling. This pathway leads to transcription of genes involved in immune response, cell adhesion, and proliferation. Co-localization studies show CARD10 forming punctate cytoplasmic complexes at the plasma membrane following receptor stimulation.Structurally, CARD10 contains an N-terminal CARD domain that mediates homotypic interactions with other CARD-containing proteins, a coiled-coil domain for oligomerization, and a C-terminal MAGUK-like domain that anchors it to membranes. It belongs to the CARMA protein subfamily, which also includes CARD11 (CARMA1) and CARD14 (CARMA2), each mediating tissue-specific NF-kappaB activation. Known interaction partners include BCL10, MALT1, and IKKgamma, forming the CBM signalosome responsible for transcriptional activation of inflammatory genes.Functionally, CARD10 plays a central role in signal transduction from GPCRs to NF-kappaB and JNK pathways. It regulates cytokine production, apoptosis resistance, and epithelial barrier integrity. In epithelial cells, CARD10-mediated signaling contributes to immune surveillance and tissue remodeling, while in vascular endothelial cells, it supports cytokine-induced adhesion molecule expression. CARD10 also regulates responses to hormones, growth factors, and stress stimuli through activation of MAPK and PI3K pathways.Dysregulation of CARD10 signaling contributes to cancer, chronic inflammation, and cardiovascular diseases. Overexpression enhances NF-kappaB-dependent transcription, promoting tumor growth and resistance to apoptosis. Mutations or altered expression of CARD10 have been reported in colorectal, breast, and renal cancers. Pathway associations include NF-kappaB signaling, GPCR signaling, and innate immune regulation. Developmentally, CARD10 expression is detected in epithelial progenitors and contributes to tissue differentiation.The CARD10 antibody from NSJ Bioreagents is a powerful reagent for research on signal transduction, NF-kappaB activation, and inflammation-related diseases.