Anti-Carbamylated Alpha-1 Antitrypsin (K359)

Alpha-1 antitrypsin is a serine protease inhibitor and member of the serpin superfamily. It has a five-stranded A beta-sheet and a mobile reactive center loop that acts as a pseudosubstrate for various proteases. Alpha-1 antitrypsin binds to a protease, undergoes proteolytic cleavage, and forms a covalent linkage between a carboxyl group in the reactive loop and the serine hydroxyl of the protease active site, effectively inactivating the enzyme which is then cleared from circulation. The primary targets of alpha-1 antitrypsin are neutrophil elastase and proteinase 3, however, it also inhibits trypsin, kallikreins 7 and 14, and matriptase. Alpha-1 antitrypsin protects the lower respiratory tract from proteolytic destruction via inhibition of neutrophil elastase and reduced serum levels of alpha-1 antitrypsin have been linked to early-onset liver disease and emphysema. Alpha-1 antitrypsin is an acute-phase protein that reduces production of inflammatory cytokines, inhibits apoptosis, blocks leukocyte degranulation and migration, as well as suppresses NF-kappaB nuclear translocation in monocytes. It delays disease onset in mouse models of inflammatory disease, including collagen-induced arthritis and experimental autoimmune encephalomyelitis (EAE). Alpha-1 antitrypsin is subject to post-translational modifications such as glycosylation and carbamylation in vivo. Carbamylated alpha-1 antitrypsin has been found in synovial fluid samples from rheumatoid arthritis patients and is predicted to act as an autoantigen. Cayman's Carbamylated Alpha-1 Antitrypsin (K359) Polyclonal Antibody can be used for Western blot applications. This antibody recognizes carbamylated alpha-1 antitrypsin at ~47 kDa from human samples.Synonyms: Carbamylated A1AT, Carbamylated AAT, Alpha-1 Antitrypsin (Homocitrulline 359), Carbamylated alpha1-Antitrypsin. Immunogen: Synthetic peptide surrounding homocitrulline 359 (335 of the mature enzyme) of human alpha-1 antitrypsin. Formulation: (Request formulation change), 500 µl of peptide affinity-purified polyclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Rabbit. Applications: WB. Cross Reactivity: (+) Carbamylated A1AT, (-) Non-carbamylated proteins. UniProt Accession #: P01009.