Anti-CALB1 / Calbindin, clone rKR6

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide. CALB1 antibody, also known as Calbindin antibody, recognizes Calbindin, a calcium-binding protein encoded by the CALB1 gene and commonly referred to as Calbindin-D28k in the literature. Calbindin belongs to the EF-hand calcium-binding protein family and functions as an intracellular calcium buffer that modulates calcium signaling dynamics in excitable and secretory cells. The protein is predominantly localized to the cytoplasm, where it binds calcium ions through multiple EF-hand domains, thereby regulating intracellular calcium homeostasis and protecting cells from calcium-mediated cytotoxicity.Calbindin is highly expressed in specific neuronal populations, particularly in cerebellar Purkinje cells, cortical interneurons, and hippocampal neurons. It is also detected in endocrine tissues, including pancreatic islets and certain epithelial cell populations in the kidney and intestine. In these contexts, Calbindin contributes to calcium-dependent signaling cascades that influence neurotransmission, hormone secretion, and epithelial transport processes. CALB1 antibody is widely used by researchers to investigate calcium buffering mechanisms, neuronal subtype identification, and tissue-specific expression patterns in both normal physiology and disease models.Calbindin-D28k plays a critical role in shaping intracellular calcium transients by binding free Ca2+ and facilitating calcium diffusion within the cytosol. Through this buffering activity, Calbindin modulates synaptic plasticity, neuronal excitability, and resistance to calcium overload. In the cerebellum, its expression in Purkinje cells makes it a well-established marker for these neurons, while in the hippocampus and cortex it marks distinct interneuron subtypes. Alterations in CALB1 expression have been associated with neurodegenerative conditions, including Alzheimer's disease and Parkinson's disease, where disrupted calcium homeostasis contributes to neuronal vulnerability. In addition, changes in Calbindin levels have been reported in certain cancers and metabolic disorders, highlighting its broader biological relevance beyond the nervous system.Structurally, Calbindin contains six EF-hand motifs, four of which are functional calcium-binding sites. The protein undergoes conformational changes upon calcium binding, enabling interactions with target proteins and influencing downstream signaling pathways. CALB1 is located on human chromosome 8q21, and its expression is developmentally regulated, with dynamic patterns observed during embryogenesis and postnatal brain maturation. Given its stable and cell type-restricted expression, CALB1 antibody serves as a reliable tool for identifying specific neuronal populations and studying calcium-dependent cellular processes. Clone KR6 is designed to recognize Calbindin in research applications and can be used to detect Calbindin expression in diverse experimental settings where calcium signaling and neuronal characterization are of interest.