Anti-c-Phospholipase A2 (Cytosolic PLA2, cPLA2)

Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and the release of arachidonic acid. cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity. Calcium binding to the Nterminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane. Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at serine 505 and serine 727 stimulates its catalytic activity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.