Anti-BubR1 (BUB1beta, BUB1B, Bub1A, hBUBR1, MAD3L, MAD3/BUB1-related Protein Kinase, Mitotic Checkpo

The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2 and Cdc20, controls chromosome segregation and monitors kinetochoremicrotubule interactions (1). This complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/ Cyclosome (APC/C) during mitosis to prevent cells with unaligned chromosomes from prematurely entering anaphase (2). Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6) and is required for functional microtubule-kinetochore interactions (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.