Anti-BUB3 (BUB3L, Budding uninhibited by benzimidazoles 3, HGNC:1151)

Bub3 (Budding uninhibited by benzimidazoles 3 homolog, also Mitotic checkpoint protein Bub3) is a 35-40kD member of the WD repeat Bub3 family of molecules. It plays a key role in chromosome segregation by serving as a component of the mitotic checkpoint complex. This complex inhibits Cdc20, and E3 ubiquitin ligase that initiates the metaphase to anaphase , transition, until microtubules are properly aligned with, and bound to, chromatid kinetochores. Human Bub3 is 328aa in length. It contains five WD40 repeats (aa5-43, 46-83, 86-124, 128- 163 and 223-262) plus a phosphorylation site at Ser211 and a Lys at #216 that may form an interchain crosslink with Gly. There are two splice variants that show two or four aa deletion over the four C-terminal amino acids. Full-length human and mouse Bub3 share 99% aa identity. , , Applications: , Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml, Western Blot: 1.0ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.