Anti-BRSK1, CT (BRSK1, KIAA1811, SAD1, SADB, Serine/threonine-protein kinase BRSK1, Brain-selective

BRSK1 may be involved as a checkpoint kinase in the regulation of G2/M arrest in response to UV- or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. This protein phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. BRSK1 is partitioned between cytoplasmic and nuclear locations in the absence of DNA damage, but translocates to the nucleus in response to Uv- or MMS-induced DNA damage. BRSK1 shares significant homology with the fission yeast Cdr2, a mitosis-regulatory kinase, and Caenorhabditis elegans SAD1, a neuronal cell polarity regulator. The BRSK1 transcript is expressed ubiquitously with the highest levels of expression in brain and testis. Applications: Suitable for use in Western Blot, Immunohistochemistry, ELISA, Recommended Dilution: ELISA: 1:1,000, Western Blot: 1:100-500, Immunohistochemistry: 1:10-50, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.