Anti-Brain-Specific Angiogenesis Inhibitor 3 (BAI3)

Human BAI-3 (brain-specific angiogenesis inhibitor 3) is a 177kD, 7-transmembrane (TM) member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI-3 is 1498 amino acids (aa) in size. It contains three distinct regions, an N-terminal extracellular domain (ECD) (aa 25-883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type1 repeats (aa 296-508), and one GPS domain (aa 816-867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one alternate splice form that shows a deletion of aa 643-665. Over aa 25-880, human BAI-3 is 98% aa identical to mouse BAI-3. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: Direct, Western Blot: 1ug/ml under non-reducing conditions only., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.