Anti-BOB1 (OCAB, POU2AF1, BOB1, OBF1, OCAB, OBF-1, POU domain, class 2, associating factor 1)

Domain Information: , Gene Ontology Terms: transcription co-activator activity, cell growth and/or maintenance, humoral immune response, regulation of transcription, DNA-dependent, transcription from Pol II promoter, nucleus , Application(s): Suitable for use in ELISA, Western Blot. Other applications not tested., Western blot: Approx 30kD band seen in Mouse Spleen lysate (Predicted MW of 28kD according to NP_006226). Recommended for use at 1-3ug/ml. Staining (2ug/ml) of Mouse Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See B2525-01-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.