Anti-BAG-1 Rabbit Monoclonal Antibody (Clone RM356)

Bcl-2-associated athanogene 1 (BAG-1) is a co-chaperone protein and member of the BAG family of proteins that has anti-apoptotic activity. It is composed of an N-terminal ubiquitin-like (UBL) domain and a conserved C-terminal BAG domain. BAG-1 has four isoforms, with the short (BAG-1S), medium (BAG-1M), long (BAG-1L) isoforms generated via alternative translation initiation sites and a BAG-1 isoform generated by post-translational modification. BAG-1L contains a nuclear localization signal in addition to the UBL and BAG domains and is the only isoform not found primarily in the cytoplasm. All BAG-1 isoforms are expressed ubiquitously and interact the anti-apoptotic protein Bcl-2 to increase its activity. Under cell stress conditions, BAG-1 binds to the ATPase domain of Hsc70 or Hsp70 with its BAG domain and inhibits DNA synthesis and cell cycling. BAG-1 interacts with additional signaling proteins, including Raf-1, which activates ERK signaling and induces cell proliferation. Protein levels of BAG-1 are increased in patient-derived invasive breast carcinoma tissue but, in contrast, its expression is positively associated with increased survival in early-stage breast cancer patients. Cayman's BAG-1 Rabbit Monoclonal Antibody (Clone RM356) can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the short, medium, and long isoforms of BAG-1 from human samples.Synonyms: BAG Family Molecular Chaperone Regulator 1, Bcl-2-associated Athanogene 1. Immunogen: Peptide corresponding to human BAG-1. Clone Designation: RM356. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC, WB. Cross Reactivity: (+) BAG-1S, (+) BAG-1M, (+) BAG-1L.