Anti-BACE1 (Beta-site Amyloid Precursor Protein Cleaving Enzyme 1, Beta-site APP Cleaving Enzyme 1,

beta-secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-beta (sAPPbeta) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99aa (CTF99). Additional processing of CTF99 by gamma-secretase generates the amyloid beta-peptide (Abeta) that forms aggregates in the brains of Alzheimer disease (AD) patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (1). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (2). BACE1 functions as a dimer and is phosphorylated at Ser498 by casein kinase 1, which is necessary for recycling back to the membrane from the early endosome (3,4). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. , Immunoprecipitation: 1:50, Immunofluorescence (frozen): 1:100, Immunofluorescence (IF-IC): 1:400, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.