Anti-b-Actin / Beta Actin

0.5mg/ml if reconstituted with 0.2ml sterile DI water. b-Actin (ACTB) is one of six different isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal isoforms. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al.(1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped four ACTB pseudogenes to other chromosomes. Karakozova et al.(2006) found that arginylation of b-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al.(2006)concluded that arginylation of b-actin apparently represents a critical step in the actin N-terminal processing needed for functioning in vivo.