Anti-ATG7 (APG7L, Ubiquitin-like Modifier-activating Enzyme ATG7, ATG12-activating Enzyme E1 ATG7, A

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. A member of the autophagy family of proteins is APG7L which was identified in yeast as a ubiquitin-E1-like enzyme, this function is conserved in the mammalian homolog. In mammalian cells, APG7L is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. The protein thus plays an indispensable role in the initial step of the conjugation system. APG7L interacts with Apg8p/Aut7p and Aut1p:Apg3p in addition to Apg12p. The protein maps to 3p25.3-p25.2 region of human chromosome. Ablation of the protein leads to abnormal swellings and dystrophy of purkinje cell axon terminals in the deep cerebellar nuclei (DCN). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml, Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.