Anti-ATG4D (ATG4 autophagy related 4 homolog D, PG4D, APG4-D, AUTL4, AUT-like 4 cysteine endopeptida

Anti-ATG4D (ATG4 autophagy related 4 homolog D, PG4D, APG4-D, AUTL4, AUT-like 4 cysteine endopeptida
Item number Size Datasheet Manual SDS Delivery time Quantity Price
A2298-74V6.100 100 µg - -

3 - 19 business days*

529.00€
 
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed... more
Product information "Anti-ATG4D (ATG4 autophagy related 4 homolog D, PG4D, APG4-D, AUTL4, AUT-like 4 cysteine endopeptida"
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA:1:32000, Western Blot:1ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Human Skeletal Muscle lysates , Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See A2298-74V6-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Keywords: Anti-APG4D, EC=3.4.22.-, Anti-Cysteine protease ATG4D, Anti-AUT-like 4 cysteine endopeptidase, Anti-Autophagy-related protein 4 homolog D, Anti-Autophagy-related cysteine endopeptidase 4
Supplier: United States Biological
Supplier-Nr: A2298-74V6

Properties

Application: ELISA, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Goat
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide, CSQLAQPTLRLPRT from the internal region (near C-terminus) of human ATG4D.
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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