Anti-ARL3 / ADP-ribosylation factor-like protein 3

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. The ARL3 antibody targets ADP-ribosylation factor-like protein 3, a small GTP-binding protein encoded by the ARL3 gene. ADP-ribosylation factor-like protein 3 is a member of the Arf family of GTPases that regulates trafficking of lipid-modified proteins to primary cilia and photoreceptor outer segments. Through its role in ciliary cargo transport, ARL3 ensures the correct localization of proteins essential for sensory signaling and phototransduction. The ARL3 antibody provides a reliable reagent for studying ciliary trafficking, GTPase signaling, and inherited retinal disorders.ADP-ribosylation factor-like protein 3 cycles between GTP- and GDP-bound states, regulating the release of lipidated cargo from carrier proteins such as PDE6D and UNC119. The ARL3 antibody supports studies that monitor these molecular interactions and visualize ARL3 localization within cilia, photoreceptor cells, and centrosomes. Its activation is mediated by the guanine nucleotide exchange factor ARL13B, while GTP hydrolysis is stimulated by the GTPase-activating protein RP2. Together, these interactions ensure precise spatiotemporal control of cargo delivery.Mutations in the ARL3 gene cause Joubert syndrome and cone-rod dystrophy, disorders associated with ciliary dysfunction and photoreceptor degeneration. The ARL3 antibody is critical for studying these ciliopathies, enabling detection of expression changes and subcellular mislocalization of mutant proteins. Disruption of ARL3-mediated trafficking leads to accumulation of mislocalized proteins and progressive loss of photoreceptor function.Beyond the retina, ADP-ribosylation factor-like protein 3 regulates microtubule organization and vesicle transport in other ciliated and non-ciliated cells. The ARL3 antibody supports investigations into these broader cellular functions, including roles in signal transduction and organelle positioning. ARL3 also contributes to lipid modification cycles that control the targeting of myristoylated and prenylated proteins.The ARL3 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, displaying distinct ciliary and pericentrosomal staining consistent with its trafficking role. NSJ Bioreagents provides this antibody as a validated reagent for molecular, cellular, and vision research. By enabling precise detection of ADP-ribosylation factor-like protein 3, the ARL3 antibody advances understanding of ciliary transport mechanisms, photoreceptor maintenance, and GTPase-mediated signaling pathways.