Anti-ARHGEF12 / Rho guanine nucleotide exchange factor 12

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. ARHGEF12 antibody detects Rho guanine nucleotide exchange factor 12, encoded by the ARHGEF12 gene on chromosome 11q23.3. ARHGEF12 antibody is widely used to study this Rho GTPase activator, also known as LARG (Leukemia-associated Rho guanine nucleotide exchange factor). ARHGEF12 belongs to the Dbl family of guanine nucleotide exchange factors (GEFs), which activate Rho proteins by promoting GDP-GTP exchange. ARHGEF12 is expressed in hematopoietic cells, endothelial cells, and many other tissues where cytoskeletal dynamics and signaling are tightly regulated.Structurally, ARHGEF12 contains several modular domains: a Dbl homology (DH) domain for GEF activity, a pleckstrin homology (PH) domain for phosphoinositide binding, and regulator of G protein signaling (RGS) motifs that mediate interaction with G alpha subunits. These structural features allow ARHGEF12 to couple G protein-coupled receptor (GPCR) signaling to RhoA activation. LARG was originally identified through its involvement in chromosomal translocations in acute myeloid leukemia, giving rise to its alias.Functionally, ARHGEF12 activates RhoA, regulating actin cytoskeleton remodeling, stress fiber formation, and cell adhesion. It plays roles in endothelial barrier function, smooth muscle contraction, and migration. ARHGEF12 also integrates signals from GPCRs, receptor tyrosine kinases, and integrins. Knockdown studies show its necessity for RhoA activation downstream of G alpha12 and G alpha13 proteins. Researchers use ARHGEF12 antibody to explore cytoskeletal dynamics, GPCR signaling, and cancer biology.Clinically, ARHGEF12 has strong links to leukemia, as its involvement in chromosomal translocations was discovered in AML. Beyond hematologic malignancies, altered ARHGEF12 expression has been implicated in solid tumors, where it influences migration and invasion. In cardiovascular biology, ARHGEF12 regulates vascular tone and contributes to hypertension. Variants in ARHGEF12 have also been associated with inherited cardiac conditions. NSJ Bioreagents supplies ARHGEF12 antibody to support studies of Rho signaling, oncogenesis, and vascular biology.Experimentally, ARHGEF12 antibody is used in western blotting to detect the ~180 kDa protein, in immunofluorescence microscopy to study actin remodeling, and in immunohistochemistry to analyze tissue distribution. Immunoprecipitation with ARHGEF12 antibody identifies interactions with G alpha subunits and cytoskeletal proteins.