Anti-ARG1 (Arginase 1) (PE)

Arginase 1 (ARG1) is a 35-40kD member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human ARG1 is 322 amino acids (aa) in length. Its enzyme region comprises aa9-309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105kD homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aainsertion after Gln43, and another that shows a deletion of aa204-289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1. By direct ELISA and western blot, this antibody detects human, mouse, and rat ARG1. Applications: Suitable for use in direct ELISA, Western Blot and Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 10ul per 1x10e6 cells using HepG2 human hepatocellular carcinoma cell line and Hepa 1-6 mouse hepatoma cell line., Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.