Anti-APRIL, mAb (Heaty-1)

Source/Host: Purified from concentrated hybridoma tissue culture supernatant. Immunogen/Antigen: Recombinant mouse APRIL. Application: ELISA: Detection Antibody Immunoprecipitation (1:200): Immunoprecipitates mouse and human APRIL and APRIL/BAFF heteromers. Crossreactivity: Human Mouse Specificity: Recognizes mouse and human recombinant and endogenous APRIL. Purity: >95% (SDS-PAGE) Purity Detail: Protein G-affinity purified. Concentration: 1mg/ml Formulation: Liquid. In PBS containing 0.02% Proclin 300. Isotype Negative Control: Mouse IgG2b Isotype Control. APRIL is a secreted cytokine, belonging to the TNF superfamily that binds to transmembrane activator and CAML interactor (TACI), B cell maturation antigen (BCMA) and can interact with carbohydrate side chains of heparan sulfate proteoglycans (HSPG) that may trigger cross-linking. APRIL maintains B cell homeostasis by acting at a later stage, modulating the function and survival of antigen-experienced B cells. APRIL (as well as BAFF) stimulates class-switch recombination (CSR), hence contributes to shaping humoral effector mechanisms. With regards to humoral memory, APRIL is involved in the establishment and survival of the long-lived plasma cell (LLPC) pool in the bone marrow (BM). Recently, APRIL has been shown to confer atheroprotection via binding to heparan sulfate (HS) chains of heparan-sulfate proteoglycan 2 (HSPG2), which limits LDL retention,macrophage accumulation and necrotic core formation. Product References Kinetics of free and ligand-bound atacicept in human serum: M. Eslami, et al., Front. Immunol. 13, 1035556 (2022)