Anti-APOBEC3C

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. APOBEC3C antibody detects DNA dC-to-dU-editing enzyme APOBEC-3C, a cytidine deaminase involved in antiviral defense, DNA mutagenesis, and innate immune regulation. Encoded by the APOBEC3C gene on chromosome 22q13.1, this enzyme belongs to the APOBEC3 subfamily of cytidine deaminases, which act by deaminating cytidine to uridine in single-stranded DNA. APOBEC3C plays a central role in restricting viral replication, particularly retroviruses and retrotransposons, through cytidine deamination and interference with reverse transcription.Structurally, APOBEC3C contains a single catalytic zinc-dependent deaminase domain characterized by the H-X-E-X(23-28)-P-C-X2-C motif, which coordinates zinc ions for catalytic activity. It primarily targets cytosines within the 5'-TC context of viral DNA, leading to G-to-A hypermutations that inhibit viral genome integrity. APOBEC3C acts against human immunodeficiency virus type 1 (HIV-1) and other retroelements by editing viral cDNA during replication. Its antiviral activity is antagonized by viral infectivity factor (Vif) proteins that mediate APOBEC degradation via the ubiquitin-proteasome pathway.The APOBEC3C antibody is used in virology, immunology, and genomic stability research to study intrinsic antiviral immunity and cytidine deamination mechanisms. Western blot analysis detects a 23 kilodalton band corresponding to APOBEC3C, while immunofluorescence shows cytoplasmic and nuclear localization depending on infection or stress conditions. This antibody supports the investigation of APOBEC3C-mediated mutagenesis, viral restriction, and immune regulation.Beyond antiviral defense, APOBEC3C influences genomic diversity and cancer mutagenesis by contributing to localized DNA editing events. Dysregulation or overexpression of APOBEC family enzymes, including APOBEC3C, has been linked to mutational signatures in various cancers. The APOBEC3C antibody provides a reliable tool for exploring the dual roles of APOBEC3C in immunity and genome evolution. NSJ Bioreagents offers this antibody validated for western blotting, immunohistochemistry, and immunofluorescence, ensuring robust and reproducible detection in studies of antiviral defense and mutation biology.