Anti-ApoB

ApoB is an amphiphilic lipid carrier protein required as the structural component for atherogenic lipoproteins. It is expressed as two major forms: a truncated isoform, ApoB-48, which is expressed in the intestines, and full-length ApoB-100, which is expressed in the liver and to a lesser extent by cardiomyocytes. ApoB-100 is composed of betaalpha1, beta1, alpha2, beta2, and alpha3 domains where the beta domains are involved in forming irreversible bonds to the lipid core and the alpha domains have reversible lipid affinity. ApoB-48 is incorporated into chylomicrons and their remnants whereas ApoB-100 is incorporated into lipoproteins VLDLs, IDLs, and LDLs. Uptake of ApoB-containing lipoproteins is primarily mediated via the LDL receptor (LDLR), heparin sulfate proteoglycans, and scavenger receptor BI (SR-BI). Transgenic mice expressing human APOB and APOA fed an atherogenic diet exhibit increased levels of VLDL- and LDL-cholesterol and increased aortic lesion areas compared with non-transgenic mice. Mutations in APOB are associated with autosomal dominant hypercholesterolemia and familial hypobetalipoproteinemia. Cayman's ApoB Polyclonal Antibody can be used for immunoprecipitation (IP) and Western blot (WB) applications. Synonyms: Apolipoprotein B. Immunogen: Purified human ApoB protein. Formulation (Request formulation change): This vial contains 500 µl of goat polyclonal antibody at 2 mg/ml. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Goat. Applications: IP, WB. Cross Reactivity: (+) ApoB (+) ApoB-48 (+) ApoB-100. Species Reactivity: (+) Human (-) Mouse. UniProt Accession No: P04114. Storage: -20°C. Stability: > 3 years.