Anti-Amyloid beta A3 Precursor Protein (APBA3, X11- , Adaptor protein X11 gamma, Mint3, X11L2)

X11-gamma (Adaptor protein X11 gamma, also APBA3, Mint3 and X11L2) is a member of the X11 family of adaptor proteins. Unlike X11-alpha and -beta which are generally neuronal proteins, X11-gamma is widely expressed in all tissues. X11-gamma has many functions, including MT-MMP and Furin intracellular trafficking and APP binding. Human X11-gamma is 575 amino acids (aa) in length. It contains a poly-Ser segment (aa165-171), a PID region (aa217-381) that binds beta-amyloid , precursor, and two PDZ domains (aa393-478 and 485-560) that bind Arf GTPases. There is one potential splice variant that shows a 34 aa insertion after Ile505. Over aa1-138, human X11-gamma shares 62% aa identity with mouse X11-gamma. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml, Western Blot:1ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and , immunoblotted with 1ug/ml sheep anti-human APBA3. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.02% sodium azide. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.