Anti-AIRE (APECED, APS1, APSI, Autoimmune Regulator, PGA1, Autoimmune Polyendocrinopathy Candidiasis

AIRE (Autoimmune Regulator, also APECED) is a 58-60kD member of the PHD Zn-finger family of transcription factors. It appears to have restricted expression, being identified in thymic medullary epithelium, dendritic cells and neutrophils. AIRE regulates the expression of self-antigens in thymus. In particular, genes coding for these antigens are generally inactive, and show minimal H3K4me3 and AcH3 reactivity in their promoters. Human AIRE is 545aa in length. It contains multiple domains, including an HSR domain that mediates homodimerization (aa1-105), an NLS (aa131-133), a SAND domain (aa181-280), and two Zn-finger PHD domains (aa296-343 and 434-475) that bind to nonmethylated histone H3. There are isoforms that show a Trp substitution for aa437-467, a 95aa substitution for aa1-292, and a 75aa substitution for aa377-545. Over aa476-545, human AIRE shares 71% aa identity with mouse AIRE. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml, Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.