Anti-Adipsin

Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology. Adipsin is a serine protease that is secreted by adipocytes. It is deficient in several animal model of obesity. Adipsin has now been identified as the same protein as complement factor D. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot:1-5 ug/ml using ECL. Adipsin is ~22 kD, ELISA: 1:10,000-50,000. Coat 10-100ng of A0890-01 control peptide/well., Optimal dilutions to be determined by the researcher. Control Peptide: A0890-02: Adipsin, Human (Control Protein for Western Blot), A0890-01: Adipsin (Blocking Peptide, ELISA Standard), Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.