Anti-ADIPOQ (ACDC, ADPN, apM-1, APM1, ACRP30, adiponectin, GBP28, adiponectin precursor, Gelatin-bin

ADIPOQ(Adipocyte-, C1q-, and Collagen Domain-Containing), also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al. (2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al. (1999) showed that APM1 is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, they found links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Adiponectin is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.1-0.5ug/ml, Western Blot: 0.1-0.5ug/ml, Immunohistochemistry (Fozen/paraffin): 0.5-1ug/ml. Antigen retrieval by boiling paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is required for the staining of paraffin sections., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.