Anti-ADAR / Adenosine deaminase acting on RNA, clone AFCB-1

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. ADAR antibody targets Adenosine deaminase acting on RNA, an RNA-editing enzyme encoded by the ADAR gene that catalyzes the conversion of adenosine to inosine within double-stranded RNA substrates. This post-transcriptional modification alters RNA sequence information and can influence RNA stability, splicing, localization, and translation. Adenosine deaminase acting on RNA is a central regulator of RNA editing events that expand transcriptomic diversity and fine-tune gene expression across many cell types.Adenosine deaminase acting on RNA contains conserved double-stranded RNA-binding domains at its N-terminus and a C-terminal deaminase catalytic domain. These domains enable selective binding to structured RNA regions and catalysis of deamination reactions. RNA editing mediated by ADAR can result in codon changes, modification of regulatory RNA elements, or alteration of microRNA processing. A short functional summary is that ADAR functions as a key enzymatic editor that modulates RNA sequence and function after transcription, thereby shaping protein diversity and regulatory RNA networks.ADAR is broadly expressed in mammalian tissues, with particularly high expression reported in the brain, immune cells, and epithelial tissues. Subcellular localization is primarily nuclear, although cytoplasmic distribution has also been observed depending on cell type and activation state. Expression levels and activity of ADAR are dynamically regulated in response to developmental cues and cellular stress signals, including interferon signaling pathways, reflecting its role in innate immune regulation and RNA surveillance.From a biological and disease relevance perspective, dysregulation of ADAR-mediated RNA editing has been linked to neurological disorders, autoimmune diseases, and cancer. Altered RNA editing patterns can disrupt normal gene function and contribute to aberrant signaling pathways. In immune contexts, ADAR activity helps distinguish self from non-self RNA, preventing inappropriate activation of antiviral responses. These diverse roles highlight the importance of precise control over ADAR expression and enzymatic activity in maintaining cellular homeostasis.An ADAR antibody is a useful research tool for detecting adenosine deaminase acting on RNA expression and studying RNA editing mechanisms in cellular and tissue-based systems. Detection of ADAR supports investigations into RNA processing, transcriptome regulation, innate immune signaling, and disease-associated changes in RNA editing landscapes. This antibody targets adenosine deaminase acting on RNA for use in research applications focused on post-transcriptional gene regulation and RNA biology.