Anti-ADAMTS 4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4) (Biot

ADAMTS4, also known as aggrecanase-1, is a secreted member of the ADAM family of proteins. ADAMTS4 is upregulated by TNF-alpha in osteoarthritis and contributes to joint destruction. Its upregulation in some cancers may enhance tumor invasiveness. Human ADAMTS4 consists of a signal peptide (aa1-51), a pro domain (aa52-212) and a mature chain (aa213-837) containing the following domains: catalytic (aa218-425), disintegrin (aa437-519), TSP type-1 (aa520-575), Cys-rich (aa 577 to 685) and spacer (aa686-837). The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human ADAMTS4 is ~5ng/lane under non-reducing and reducing conditions., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.