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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
0014-07A.100 | 100 µg | - | - |
3 - 19 business days* |
1,212.00€
|
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You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also... more
Product information "Anti-14-3-3 sigma, clone 1.N.6"
14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 proteins form homo- and heterodimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner. 14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1 and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. Expression of 14-3-3 sigma is higher in normal epithelial cells. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 2ug/mg protein lysate. Use Protein G., Western Blot: 1ug/ml for 2hrs at RT., Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes., Optimal dilutions to be determined by the researcher. Positive Control: HT29 cells, Skin, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | Anti-SFN, Anti-HME1, Anti-Stratifin, Anti-14-3-3 protein sigma, Anti-Epithelial cell marker protein 1 |
Supplier: | United States Biological |
Supplier-Nr: | 0014-07A |
Properties
Application: | IF, IHC, IP, WB |
Antibody Type: | Monoclonal |
Clone: | 1.N.6 |
Conjugate: | No |
Host: | Mouse |
Species reactivity: | human |
Immunogen: | human |
Format: | Affinity Purified |
Database Information
KEGG ID : | K06644 | Matching products |
UniProt ID : | P31947 | Matching products |
Gene ID | GeneID 2810 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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