Anti-mCherry Nanobody Affinity Agarose

Coupled ligand: Anti-mCherry nanobody. Beads: Sepharose 4B agarose with average particle size of 50 µm. Binding capacity: 1.2mg protein /ml agarose. Storage buffer: PBS with preservative and 50% glycerol, pH7.2. Storage conditions: -20? for 12 months. Background: mCherry, a red fluorescent protein derived from sea anemone (Discosoma sp.), is composed of 266 amino acids. Because of its color and light stability of monomer molecules, it performs better than other fluorescent protein tags. mCherry is often used as eukaryotic protein recombinant expression marker. Anti-mCherry nanobody affinity gel is made by covalently coupling high-quality mCherry nanobody with agarose gel. As the nanobody only contains the variable region of antibody molecules, there will be no signal interference from the heavy and light chains of the antibody during immuno (co) precipitation. It has the characteristics of high binding capacity of protein, high specificity and stability, and can be used for immuno (co) precipitation of mCherry tagged fusion protein.