The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HEPH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HEPH. Next,...

Protein function: May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1. [The UniProt Consortium] Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Hephaestin (HEPH) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of HEPH in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 1.56-100ng/ml, Sensitivity: 0.54ng/ml, Storage and Stability: Desiccation recommended. The Standard,...

Protein function: May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1. [The UniProt Consortium] Buffer: PBS and 1M Urea, pH 7.4.