Strep-Tactin(R) TACS Agarose

Strep-Tactin(R) TACS Agarose contains agarose macrobeads coated with the streptavidin variant Strep-Tactin(R). A special crosslinking permits the use for cell isolation and depletion approaches. It was developed for column-based Traceless Affinty Cell Selection (Fab-TACS(R)/Nano-TACS(R)). In this approach, Fab fragments fused to a Twin-Strep-tag(R) (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin(R) on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin(R) Agarose by biotin addition. In this way, the eluted cells are ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS(R) for exosomes). Alternatively to Fab-Streps/Nano-Streps, it is possible to bind Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding) to this resin. This makes Strep-Tactin(R) TACS Agarose also suitable for negative cell selection or depletion approaches. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.