Anti-HA (YPYDVPDYA) FAST IP kit

Coupled ligand: Anti-HA-Tag mouse monoclonal antibody. Beads: Sepharose 4B agarose with average particle size of 50 µm. Binding capacity: 1.2mg protein /ml agarose. Storage buffer: PBS with preservative and 50% glycerol, pH7.2. Storage conditions: -20? for 12 months. Background: Anti-HA-tag(YPYDVPDYA) fast IP kit is composed of Anti-HA affinity gel, Mouse IgG affinity gel, HA-Tag Rabbit pAb and Goat Anti-Rabbit IgG(peroxidase/HRP conjugated), which is used for fast, efficient and specific (Co)IP of HA tagged fusion protein. Anti-HA affinity gel is made by covalently coupling high-quality HA-Tag Mouse mAb with agarose gel. It has the characteristics of high binding capacity of protein, high specificity and stability. Mouse IgG affinity gel is used as the control for CoIP experiment. The HA-Tag Rabbit pAb antibody has the advantages of high specificity, high affinity and high valence, Goat Anti-Rabbit IgG (peroxidase/HRP conjugated) is purified by cross-adsorption and only HA-Tag Rabbit pAb is identified, and there is no cross-reaction with the heavy-chain and light-chain of the mouse monoclonal antibody. The four components are strictly inspected and can be used independently, the combination of four sets has the advantages of fast, convenience and no interference band.