Twin-Strep-tag(R) Capture Maxi Kit

Twin-Strep-tag(R) Capture Kit is intended for site-directed, reversible capture of Twin-Strep-tag(R) fusion proteins for biomolecular interaction analysis using surface plasmon resonance (SPR) devices. The kit provides Strep-Tactin(R)XT, SPR Immobilization Buffer, Regeneration Buffer, and GFP-Twin-Strep-tag(R) as control protein. It enables the highly stable immobilization of the ligand based on the unique Strep-Tactin(R)XT:Twin-Strep-tag(R) interaction, which operates with pM affinity. The key benefits are:  Indirect immobilization protecting the ligand from chemical modification as well as inactivation or manipulation by the immobilization procedure  High affinity enabling measurements over a long period (high affinity in low pM range, T1/2 = 13 h)  Chip can be regenerated easily by an established procedure - cost effective  Ligands can be captured directly from complex samples due to the high affinity and specificity of the Twin-Strep-tag(R):Strep-Tactin(R)XT interaction The use of unconjugated Strep-Tactin(R)XT requires a license that is provided with the purchase of the kit as limited use license.