NZYMutagenesis kit

NZYMutagenesis kit is designed to make point mutations and delete or insert single or multiple nucleotides in a DNA sequence. The system requires the provision of two synthetic oligonucleotide primers containing the desired mutation. Incorporation of the oligonucleotide primers with NZYProof DNA polymerase generates a mutated plasmid containing staggered nicks, which resists Dpn Idigestion (as the synthetic DNA is not methylated). The resulting mutated plasmid is recovered through transformation of NZYStar competent cells. For a schematic presentation of the mutagenesis protocol, see Figure 1. DNA isolated from dam- Escherichia coli strains, including JM101 and SCS110, is not a suitable template forthe mutagenesis reaction. Components: * Reaction Buffer (10x) * dNTP mix * NZYProof DNA polymerase (2.5 U/µL) * Dpn I (10 U/µL) * Control plasmid * Control primer mix * NZYStar Competent Cells (*) * Competent Cells Control Plasmid (0.1 ng/µL) (*)(*) only provided in MB01202 kit. Shipping Conditions: Dry Ice. Storage Conditions: Competent cells: -85 °C to -65 °C / Other components: -30 °C to -15 °C. Application: Site-directed mutagenesis. Features: Mutagenesis of plasmids <15 kb. Product Category: DNA Cloning & Mutagenesis