Buccutite(TM) Rapid PE Antibody Labeling Kit *Production Scale Optimized for Labeling 1 mg Antibody

Correction Factor (280 nm): 0.175. Extinction coefficient (cm^-1 M^-1): 1960000. Excitation (nm): 565. Emission (nm): 574. Quantum yield: 0.82. Buccutite(TM) Rapid PE Antibody Labeling Kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with PE, APC, and iFluor(R) tandem dyes. In comparison to traditional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite(TM) conjugation is more robust and straightforward. Using a two-step mixing protocol, researchers can directly conjugate PE to any antibody or protein in less than 2 hours. Each Buccutite(TM) kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column for maximum conjugate yield. Each Buccutite(TM) FOL-Activated PE vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. Phycoerythrin (PE) is an intensely bright, red-orange fluorescent phycobiliprotein with an excitation and emission maxima of ~565 nm and ~576 nm, respectively. Given its intense brightness, PE is recommended for pairing with low-abundance targets to minimize spillover and compensation. PE conjugates are well-suited for flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte(TM) Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.