Buccutite(TM) Rapid Crosslinked APC Antibody Labeling Kit *Production Scale Optimized for Labeling 1

Correction Factor (280 nm): 0.195. Extinction coefficient (cm^-1 M^-1): 730000. Excitation (nm): 651. Emission (nm): 660. Buccutite(TM) Rapid Crosslinked APC Antibody Labeling Kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with APC, PE, and iFluor(R) tandem dyes. In comparison to traditional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite(TM) conjugation is simple and more robust. Using a two-step mixing protocol, researchers can directly conjugate APC to any antibody or protein in less than 2 hours. Each Buccutite(TM) kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column to maximize conjugate yield. Each Buccutite(TM) FOL-Activated APC vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. Allophycocyanin (APC) is an intensely bright, red fluorescent phycobiliprotein with an excitation and emission maxima of ~651 nm and ~660 nm, respectively. Given its intense brightness, APC is recommended for pairing with low-abundance targets to minimize spillover and compensation. APC conjugates are well-suited for FRET screening, flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte(TM) Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.