Buccutite(TM) Rapid APC-Cy5.5 Tandem Antibody Labeling Kit *Production Scale Optimized for Labeling

Extinction coefficient (cm^-1 M^-1): 700000. Excitation (nm): 651. Emission (nm): 700. Buccutite(TM) Rapid APC-Cy5.5 Tandem Antibody Labeling Kits, designed for large-scale production, provide a streamlined approach for labeling antibodies with PE, APC, PerCP, and iFluor(R) tandem dyes. Compared to conventional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite(TM) conjugation is simple and more robust. Using a two-step mixing protocol, researchers can directly conjugate APC-Cy5.5 to any antibody or protein in less than 2 hours. Each Buccutite(TM) kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column for maximum conjugate yield. Each Buccutite(TM) FOL-Activated APC-Cy5.5 vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. Allophycocyanin-cyanine 5.5 (APC-Cy5.5) is an intensely bright, red fluorescent tandem fluorophore with an excitation and emission maxima of ~651 nm and ~700 nm, respectively. Given its intense brightness, APC-Cy5.5 is recommended for pairing with low-abundance targets to minimize spillover and compensation. APC-Cy5.5 conjugates are well-suited for flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte(TM) Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.