Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
NZY-MB12501 | 50 rxns | - |
Request delivery time estimate |
196.00€
|
|||
NZY-MB12502 | 250 rxns | - |
Request delivery time estimate |
857.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
NZY First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to... more
Product information "NZY First-Strand cDNA Synthesis Kit"
NZY First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA.The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY First-Strand cDNA Synthesis Kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA. The kit includes a combination of random hexamers and oligo(dT)18 primers in order to increase sensitivity. The primers are included in the NZYRT 2x Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H minus) and the NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA:RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reaction since PCR primers will bind more easily to the cDNA. Components: * NZYRT Enzyme Mix * NZYRT 2x Master Mix * NZY RNase H (E. coli) * DEPC-treated H2O. Shipping Conditions: Dry Ice. Storage Conditions: -85 °C to -15 °C. Application: cDNA synthesis, RT-PCR, RT-qPCR. Features: Dual-primer: oligo(dT) + random hexamers, Fast, High yield, Thermostable. Format: Kit, Liquid. Product Category: cDNA Synthesis. Reaction Temperature: 50 °C. Protocol Time: Approx. 60 min
Supplier: | NZYtech |
Supplier-Nr: | MB125 |
Properties
Application: | First-strand cDNA synthesis |
Format: | Solution |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | -20°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
Viewed