NZY-blunt PCR cloning kit

NZY-blunt PCR cloning kit was designed to allow the direct cloning of PCR products with blunt ends, which result from PCR amplifications using proofreading DNA polymerases, into pNZY28. The pNZY28 vector provided was linearized at the EcoR V and dephosphorilated. Components: * Phos Buffer (10x) * Phos Enzyme Mix * Positive control insert (120 ng/µL) * Linearized pNZY28 vector (20 ng/µL) * T4 DNA Ligase (2 U/µL) * NZYStar Competent Cells (*) * Competent Cells Control Plasmid (0.1 ng/µL) (*)(*) only provided in MB12101 kit. Shipping Conditions: Dry Ice. Storage Conditions: Competent cells: -85 °C to -65 °C-Other components: -30 °C to -15 °C. Application: Blunt-end Cloning. Features: Blue/White screening. Product Category: DNA Cloning & Mutagenesis. Protocol Time: Approx. 60 min