GenieClone DNA Assembly Cloning Kit

GenieClone DNA Assembly Cloning Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
G-MORV0004.25 25 rxns - -

9 - 20 business days*

264.00€
G-MORV0004.50 50 rxns - -

9 - 20 business days*

428.00€
 
GenieClone DNA Assembly Cloning Kit allows for the highly efficient cloning and assembly of 1-5... more
Product information "GenieClone DNA Assembly Cloning Kit"
GenieClone DNA Assembly Cloning Kit allows for the highly efficient cloning and assembly of 1-5 DNA fragments in as little as 15 minutes into any vector at any site. This system utilizes ligation-independent technology and a novel GenieClone recombinase to significantly reduce self-ligating colonies. The enhanced GenieClone Recombinase and highly optimized buffer included in the 2x GenieClone Mix significantly improve the recombination efficiency and the tolerance to impurities. The pCE-ONE vector is compatible with most PCR products, enabling the specific PCR fragments to be used directly for recombination without any pre-treatment. Utilizing a simple procedure, the vector is first linearized at the cloning site. A small overlapping sequence at each end of the cloning site is added to the insert through PCR. The insert and the linearized vector both with overlapping 5'- and 3' (15 bp - 20 bp) sequences are mixed in an appropriate ratio and incubated with GenieClone Recombinase at 50? for 5 - 15 min before transformation of competent cells for successful DNA cloning and assembly.
Supplier: Assay Genie
Supplier-Nr: MORV0004

Properties

Application: Highly efficient cloning

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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